Coding

Part:BBa_K2433001:Design

Designed by: Tamar Av-Shalom   Group: iGEM17_British_Columbia   (2017-10-25)


ietS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 425
    Illegal XhoI site found at 2020
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1330
    Illegal NgoMIV site found at 2214
    Illegal AgeI site found at 2371
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 505
    Illegal BsaI.rc site found at 1690
    Illegal BsaI.rc site found at 1972


Design Notes

We replaced some codons to remove internal cut sites.


Source

Atu6083 from pTiC58(https://www.ncbi.nlm.nih.gov/nuccore/159141737)

References